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presynthesized miscript primer assay cel-mir-39-3p  (Qiagen)


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    Qiagen presynthesized miscript primer assay cel-mir-39-3p
    Presynthesized Miscript Primer Assay Cel Mir 39 3p, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+primer+assay+cel-mir-39/cel+mir+39/pm40326038-74-0-7
    Average 90 stars, based on 1 article reviews
    presynthesized miscript primer assay cel-mir-39-3p - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Isolation:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Control:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    cDNA Synthesis:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Quantitative RT-PCR:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Polymerase Chain Reaction:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Purification:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Extraction:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).

    Ethanol Precipitation:

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency
    Article Snippet: An endogenous control (5.6 × 10 8 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: Diagnostic Potential of Exosomal HypoxamiRs in the Context of Hypoxia–Sumoylation–HypoxamiRs in Early Onset Preeclampsia at the Preclinical Stage
    Article Snippet: The samples were prepared according to the following protocol: whole blood was centrifuged at 300× g , 4 °C for 20 min, and then the supernatant was centrifuged at 16,000× g for 10 min. A total of 200 μL of the prepared plasma was used for isolation of exosomes, and 5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen, Hilden, Germany) were added to a plasma sample as an endogenous control for the efficiency of isolation and subsequent cDNA synthesis for quantitative RT-PCR.

    Article Title: Interrelation between miRNAs Expression Associated with Redox State Fluctuations, Immune and Inflammatory Response Activation, and Neonatal Outcomes in Complicated Pregnancy, Accompanied by Placental Insufficiency.
    Article Snippet: An endogenous control (5.6 × 108 copies of cel-miR-39 (miScript Primer Assay, Qiagen)) was added to a plasma sample for evaluation of the efficiency of isolation and subsequent cDNA synthesis for a quantitative RT-PCR.

    Article Title: CircEHD2, CircNETO2 and CircEGLN3 as Diagnostic and Prognostic Biomarkers for Patients with Renal Cell Carcinoma
    Article Snippet: The PCR primer for cel-miR-39 (used as reference gene) was purchased from Qiagen (miScript Primer Assay, catalog no. 218300).



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    Image Search Results


    cf-miRNAs DE in T0 plasma samples of NRs vs. Rs according to small RNA-seq data analysis.

    Journal: Cancers

    Article Title: Circulating miR-1246 and miR-485-3p as Promising Biomarkers of Clinical Response and Outcome in Melanoma Patients Treated with Targeted Therapy

    doi: 10.3390/cancers14153706

    Figure Lengend Snippet: cf-miRNAs DE in T0 plasma samples of NRs vs. Rs according to small RNA-seq data analysis.

    Article Snippet: Total RNA (5 μL) was used for cDNA synthesis and reverse transcription was carried out using the miScript II RT kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol with HiSpec buffer. qRT-PCR was performed in a final volume of 25 μL using miScript II SYBR green PCR kit (Qiagen) and the pre-validated miScript Primer Assays for hsa-miR-92b-3p (cat.# MS00032144) and for the spike-in control cel-miR-39 (cat.# MS00019789) (Qiagen) according to manufacturer’s instruction.

    Techniques: Clinical Proteomics

    ROC analysis on cf-miRNAs DE in T0 plasma samples of NRs vs. Rs according to small RNA-seq data analysis.

    Journal: Cancers

    Article Title: Circulating miR-1246 and miR-485-3p as Promising Biomarkers of Clinical Response and Outcome in Melanoma Patients Treated with Targeted Therapy

    doi: 10.3390/cancers14153706

    Figure Lengend Snippet: ROC analysis on cf-miRNAs DE in T0 plasma samples of NRs vs. Rs according to small RNA-seq data analysis.

    Article Snippet: Total RNA (5 μL) was used for cDNA synthesis and reverse transcription was carried out using the miScript II RT kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol with HiSpec buffer. qRT-PCR was performed in a final volume of 25 μL using miScript II SYBR green PCR kit (Qiagen) and the pre-validated miScript Primer Assays for hsa-miR-92b-3p (cat.# MS00032144) and for the spike-in control cel-miR-39 (cat.# MS00019789) (Qiagen) according to manufacturer’s instruction.

    Techniques: Clinical Proteomics

    T0 plasma level of cf-miRNAs according to PFS categories.

    Journal: Cancers

    Article Title: Circulating miR-1246 and miR-485-3p as Promising Biomarkers of Clinical Response and Outcome in Melanoma Patients Treated with Targeted Therapy

    doi: 10.3390/cancers14153706

    Figure Lengend Snippet: T0 plasma level of cf-miRNAs according to PFS categories.

    Article Snippet: Total RNA (5 μL) was used for cDNA synthesis and reverse transcription was carried out using the miScript II RT kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol with HiSpec buffer. qRT-PCR was performed in a final volume of 25 μL using miScript II SYBR green PCR kit (Qiagen) and the pre-validated miScript Primer Assays for hsa-miR-92b-3p (cat.# MS00032144) and for the spike-in control cel-miR-39 (cat.# MS00019789) (Qiagen) according to manufacturer’s instruction.

    Techniques: Clinical Proteomics

    Box-and-whisker diagrams of miR-1246, mir-92b-3p and miR-485-3p levels in melanoma patients treated with targeted therapy. ( A ). cf-miRNA levels were measured by qRT-PCR in plasma samples obtained from 55 patients (45 Rs and 10 NRs) before the start of therapy (T0). ( B ). cf-miRNA levels were measured by qRT-PCR in matched plasma samples obtained from 34 patients at T0 and at progression (TP). The edges of each box represent the 75th and 25th percentile, respectively, and whiskers are defined according to Tukey method. The horizontal bar within each box indicates the median. The outliers are reported as dots. Relative expression levels of miR-485-3p and miR-92b-3p are multiplied by 100. Data were analyzed by nonparametric Mann–Whitney U test to compare differences between groups, ( A ) and by the Wilcoxon matched-pairs signed-rank test for the before–after differences ( B ). ** p < 0.01 and * p < 0.05.

    Journal: Cancers

    Article Title: Circulating miR-1246 and miR-485-3p as Promising Biomarkers of Clinical Response and Outcome in Melanoma Patients Treated with Targeted Therapy

    doi: 10.3390/cancers14153706

    Figure Lengend Snippet: Box-and-whisker diagrams of miR-1246, mir-92b-3p and miR-485-3p levels in melanoma patients treated with targeted therapy. ( A ). cf-miRNA levels were measured by qRT-PCR in plasma samples obtained from 55 patients (45 Rs and 10 NRs) before the start of therapy (T0). ( B ). cf-miRNA levels were measured by qRT-PCR in matched plasma samples obtained from 34 patients at T0 and at progression (TP). The edges of each box represent the 75th and 25th percentile, respectively, and whiskers are defined according to Tukey method. The horizontal bar within each box indicates the median. The outliers are reported as dots. Relative expression levels of miR-485-3p and miR-92b-3p are multiplied by 100. Data were analyzed by nonparametric Mann–Whitney U test to compare differences between groups, ( A ) and by the Wilcoxon matched-pairs signed-rank test for the before–after differences ( B ). ** p < 0.01 and * p < 0.05.

    Article Snippet: Total RNA (5 μL) was used for cDNA synthesis and reverse transcription was carried out using the miScript II RT kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol with HiSpec buffer. qRT-PCR was performed in a final volume of 25 μL using miScript II SYBR green PCR kit (Qiagen) and the pre-validated miScript Primer Assays for hsa-miR-92b-3p (cat.# MS00032144) and for the spike-in control cel-miR-39 (cat.# MS00019789) (Qiagen) according to manufacturer’s instruction.

    Techniques: Whisker Assay, Quantitative RT-PCR, Clinical Proteomics, Expressing, MANN-WHITNEY